Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTLYTSPSCTSCRKARAWLEEHEIPFVERNIFSEPLSIDEIKQILRMTEDGTDEIISTRSKVFQKLNVNVESMPLQDLYR--LINEHPGLLRRPIIIDEKRLQVGYNEDEIRRFLPRKVRSFQLREAQRLAN 2M46 Chain:A ((1-113)) MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKTIIANTGVEINKLFNTHGAKYRELDLKNKLQTLSDDEKLELLSSDGMLVKRPLAVMGDKITLGFKEDQYK--------------------

General information: TITO was launched using: RESULT: Template: 2M46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 444 -54583 -122.93 -491.74 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -122.93 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_2M46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2M46-query.scw PDB file : Tito_Scwrl_2M46.pdb: