Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLKTKIHLYTSISLLILLILVHTAVYLIFSSALTSKDAARLADETDNIAEALRAAETEGVALQDMLQAYLPANGMVRVVNGDQKAVMTITKEKAYKDFPLSFHSGETADVRKPDGKLFAEAAVPVIWTDGQVVSLQLVERLENTEESLFLLKIILIAASAAVCIASFFAGSLLARRIINPIRRLMITMKDIQRDKEFKTISLEGQSNDELYQMGLTFNEMAMMLKEHYDKQQQFVQDASHELKTPLTIIESYS-SLMKRWGAKKPEVLEESIEAIHSEAVHMKKLTNQLLALAK-SHQGLEVDLKTIDL---IKAARAVMQTLQSVYQRDILLETDKESLL-VKADEERIKQLLTILLDNAIKYSEKP-----IEMSAGTRNGRPFLSVRDEGIGIPEEHIPHLFERFYRADEARNRKTGGTGLGLSIAKQIADEHGIELSVKSKPGQGTAVTMQFSEQNGGGR 3DGE Chain:A ((20-250)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KTEFIANISHELRTPLTAIKAYAETIYNSLGELDLSTLKEFLEVIIDQSNHLENLLNELLDFSRLERKSLQINREKVDLCDLVESAVNAIKEFASSHNVNVLFESNVPCPVEAYIDPTRIRQVLLNLLNNGVKYSKKDAPDKYVKVILDEKDGGVLIIVEDNGIGIPDHAKDRIFEQFYRVDSSLTYEVPGTGLGLAITKEIVELHGGRIWVESEVGKGSRFFVWIPKDRA---

General information: TITO was launched using: RESULT: Template: 3DGE.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 891 -88402 -99.22 -401.83 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -99.22 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_3DGE.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DGE-query.scw PDB file : Tito_Scwrl_3DGE.pdb: