Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFQSTEIGIDLGTANILVYSKNKGIILNEPSVVAVDTT--TKAVLAIGADAKNMIGKTPGKIVAVRPMKDGVIADYDMTTDLLKHIMKKAAKSIGMSFRKPNVVVCTPSGSTAVERRAISDAVKNCGAKNVHLIEEPVAAAIGADLPVDEPVANVVVDIGGGTTEVAIISFGGVVSCHSIRIGGDQLDEDIVSFVRKKYNLLIGERTAEQVKMEIGHALIEHIPEAM--EIRGRDLVTGLPKTIMLQSNEIQDAMRESLLHILEAIRATLEDCPPELSGDIVDRGVILTGGGALLNGIKEWLTEEIVVPVHVAQNPLESVAIGTGRSLEVIDKLQKAIK 4CZG Chain:A ((3-326)) ---SNDIAIDLGTANTLIYQKGKGIVLNEPSVVALRNVGGRKVVHAVGIEAKQMLGRTPGHMEAIRPMRDGVIADFEVAEEMIKYFIRKVHNRKGSG--NPKVIVCVPSGATAVERRAINDSCLNAGARRVGLIDEPMAAAIGAGLPIHEPTGSMVVDIGGGTTEVAVLSLSGIVYSRSVRVGGDKMDEAIISYMRRHHNLLIGETTAERIKKEIGTARAP----GLSIDVKGRDLMQGVPREVRISEKQAADALAEPVGQIVEAVKVALEATPPELASDIADKGIMLTGGGALLRGLDAEIRDHTGLPVTVADDPLSCVALGCGKVLE----------

General information: TITO was launched using: RESULT: Template: 4CZG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1866 -181428 -97.23 -574.14 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -97.23 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_4CZG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4CZG-query.scw PDB file : Tito_Scwrl_4CZG.pdb: