Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNNLPIIALDFASAEETLAFLAPFQQEPLFVKVGMELFYQEGPSIVKQLKERNCELFLDLKLHDIPTTVNKAMKRLASLGVDLVNVHAAGGKKMMQAALEGLEEGTPAGKKRPSLIAVTQLTSTSEQIMKDELLIEKSLIDTVVHYSKQAEESGLDGVVCSVHEAKAIYQAVSPSFLTVTPGIRMSEDAANDQVRVATPAIAREKGSSAIVVGRSITKAEDPVKAYKAVRLEWEGIKS 3UWQ Chain:A ((31-243)) ------IVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVKAAAELGVWMVNVHASGGERMMAASREILE---PYGKERPLLIGVTVLTSMESADLQG-IGILSAPQDHVLRLATLTKNAGLDGVVCSAQEASLLKQHLGREFKLVTPGIRPA-------RRIMTPAQAIASGSDYLVIGRPITQAAHP----------------

General information: TITO was launched using: RESULT: Template: 3UWQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1026 -139844 -136.30 -678.85 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -136.30 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.569

(partial model without unconserved sides chains): PDB file : Tito_3UWQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UWQ-query.scw PDB file : Tito_Scwrl_3UWQ.pdb: