Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKEVLTQTKEKMEKAIAAYQRELATVRAGRANPSLLDKVTVEYYGAQTPLNQLSSINVPEARMLVITPYDKTAIGDIEKAILKADLGLTPTSDGNMIRIAIPALTEERRKELVKVVKKYAEEAKVAVRNVRRDANDDLKKLEKNGDITEDELRASTEDVQKLTDEYVSKIDSVTKDKEKEIMEV 4KAW Chain:X ((2-184)) -IDEALFDAEEKMEKAVAVARDDLSTIRTGRANPGMFSGITIDYYGAATPITQLASINVPEARLVVIKPYEANQLRAIETAIRNSDLGVNPTNDGALIRVAVPQLTEERRRELVKQAKHKGEEAKVSVRNIRRKAMEELHRIRKEGEAGEDEVGRAEKDLDKTTHQYVTQIDELVKHKEGELLE-

General information: TITO was launched using: RESULT: Template: 4KAW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain X - contact count / total energy / energy per contact / energy per residue : 718 -76804 -106.97 -419.69 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain X : 0.89 3D Compatibility (PKB) : -106.97 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.660

(partial model without unconserved sides chains): PDB file : Tito_4KAW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KAW-query.scw PDB file : Tito_Scwrl_4KAW.pdb: