Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRVLVVDDAKFMRVKIREILEEANYIIAGEAADGEQAADLYKKLRPDLVTMDITMPVKNGIKALRDILTFDPKAKVIMCTAMRQQRIVTEAIELGAKDFIVKPFEETKVLEAVSRVMGH 1U0S Chain:Y ((4-118)) --RVLIVDDAAFMRMMLKDIITKAGYEVAGEATNGREAVEKYKELKPDIVTMDITMPEMNGIDAIKEIMKIDPNAKIIVCSAMGQQAMVIEAIKAGAKDFIVKPFQPSRVVEALNKV---

General information: TITO was launched using: RESULT: Template: 1U0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain Y - contact count / total energy / energy per contact / energy per residue : 572 -91252 -159.53 -793.49 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain Y : 0.89 3D Compatibility (PKB) : -159.53 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.706

(partial model without unconserved sides chains): PDB file : Tito_1U0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U0S-query.scw PDB file : Tito_Scwrl_1U0S.pdb: