Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYASKLKKGDEIRIVSPATSMSILSNEAKIQAKTALERLGYRVTIAEHANECNEFDSSSIESRVHDLHAAFFDPGVKAILTTLGGFNSNQLLRYLDYEKIKRHPKILCGYSDITALCNAIYQKTGLVTYSGPHF--STFAMKKGLDYTEEYFLSCCASDD--PFEIHPSSEWSDD--RWFLDQENRRFYPNNGPVVIQEGYAEGTLIGGNLCTLNLLQGTEYFPETEHTILLIEDDYMSDIHMFDRDLQSLIHLPAFSHVKAILIGRFQKASNVSI-----DLVKAMIETKKELSGIPIIANINAGHTSPIATFPIGGTCRIEAISGTSRIWIDKH 3TYX Chain:A ((10-330)) -----LKYGDTIGIYSPSSPVTYTSPKRFERAKSYLLQKGFHILEGSLTGRYDYYRSGSIQERAKELNALIRNPNVSCIMSTIGGMNSNSLLPYIDYDAFQNNPKIMIGYSDATALLLGIYAKTGIPTFYGPALVPSFGEFEPFVDDTYKYFLETLLHDQALPYNIKQPLFWSDESINWEEKTKEKELRPNNW-ISVTNGQATGRVIGGNLNTIQGIWGSPYMPCIQEGDILFIEDSSKDAATIERSFSFLKINGVFDKVSGIILGKHEQFDDCGTNRKPYEILLEVLQNQR----IPLLADFDCCHTHPMITMPIGVQVKMDATNKTIHI-----

General information: TITO was launched using: RESULT: Template: 3TYX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1856 -44433 -23.94 -143.33 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -23.94 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_3TYX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TYX-query.scw PDB file : Tito_Scwrl_3TYX.pdb: