Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDMLYWLLIAAVFIIAFIGLVYPVI-------------PSVVFIVAGFVLYGFLFSFSPYSYMFWLVEAVFAAVLFAADYVSNLLGVKRFGGSKAAIWGSTIGLLIGPFVIPVAGIILGPFIGAVCAELIVHQKDLKSAFKIGLGSLIGFLTGVIAKGIIQLLMIGYFLWTVL 2WJM Chain:M ((147-232)) -----WNFAAAIFFVLCIGCIHPTLVGSWSEGVPFGIWPHIDWLTAFSIRYG-NFYYCPW-HGFSIGFAYGCGLLFAA-HGATILAVARFGGDR-------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2WJM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 98 -26090 -266.22 -357.40 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain M : 0.50 3D Compatibility (PKB) : -266.22 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_2WJM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2WJM-query.scw PDB file : Tito_Scwrl_2WJM.pdb: