Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITQLYRERTAADLKNRISKVLLNGNETEIVELTIQGAVVTVLTQREEDIKHIKSVQILDDQNNVITERTTDLDVSNNRTLDFRITFEVV 2HQV Chain:A ((144-158)) ----------------------------------------------------SCSVWFMNAAGGAMF-----------------------

General information: TITO was launched using: RESULT: Template: 2HQV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 14 -3232 -230.82 -215.43 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -230.82 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.576

(partial model without unconserved sides chains): PDB file : Tito_2HQV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HQV-query.scw PDB file : Tito_Scwrl_2HQV.pdb: