Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALSPEIEFEDIEDDSEVIETSQTYKIDFENGRITNEIITGLEAIKQFVYLSLHTERYAYSVYSHDIGNELQDVLADNETTDAYKKMEIPRLIEEALIYDDRISA--VTDFEIEKQGESFRVSFTVETDEGTLEIEEVLGEDV 4OIW Chain:A ((135-235)) ------------------------------NGKLESRLVDFRKAIAVIPNLAIHLNRAANEGWPINAQNELPPIIAQLAPGEA---ADFRLLLDEQLLREHGITADVVLDYEL------------------------------

General information: TITO was launched using: RESULT: Template: 4OIW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 127 -6233 -49.08 -79.91 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -49.08 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.093

(partial model without unconserved sides chains): PDB file : Tito_4OIW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OIW-query.scw PDB file : Tito_Scwrl_4OIW.pdb: