Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYDTIVIGAGQAGISIGYYLK-QSDQKFIILDKSHEVGESWK-DRYDSLVLFTSRMYSSLPGMH----LEGEKHGFPSKNEIVAYLKKYVKKFEIP--IQLRTEVISVLKI--KNYFLI-KTNREEYQTKNLVIATGPFHTPNIPSISKDLSDNINQLHSSQYKNSKQLAYGNVLVVGGGNSGAQIAVELSKE-RVTYLACSNKLVYFPLMIGKRS--I-F----------WWF----------------------DKLGVLHA----------S------------H----TSIVGKF-------------IQ----KKGDPVFGHELKHAIKQKEIILK----KRVIAAKQNEIIFKDSSTLEVNNIIWATGFRNPLCWINIKGVLDQEGRIIHH----------RGVS-PVEGLYF-IGLPWQHKRGSALLQGVGNDAEYIVKQMNGE 4RG3 Chain:A ((16-462)) TVDAVVIGAGFGGIYAVHKLHHELGLTTVGFDKADGPGGTWYWNRYPGALSDTESHLYRFSFDRDLLQESTWKTTYITQPEILEYLEDVVDRFDLRRHFKFGTEVTSALYLDDENLWEVTTDHGEVYRAKYVVNAVGLLSAINFPNLPGLDTFEGETIHTAAWPEGKSLAGRRVGVIGTGSTGQQVITSLAPEVEHLTVFVRTPQYSVPVGNRPVNPEQIAEIKADYDRIWERAKNSAVAFGFEESTLPAMSVSEEERNRIFQEAWDHGGGFRFMFGTFGDIATDEAANEAAASFIRAKVAEIIEDPETARKLMPKGLFAKRPLCDSGYYEVYNRPNVEAVAIKENPIREVTAKGVVTEDGVLHELDVLVFATGFDAVDGNYRRIEIRGRDGLHINDHWDGQPTSYLGVSTANFPNWFMVLGPNGP---FTNLPPSIETQVEWISDTIGY-

General information: TITO was launched using: RESULT: Template: 4RG3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1646 9223 5.60 27.05 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : 5.60 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_4RG3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RG3-query.scw PDB file : Tito_Scwrl_4RG3.pdb: