Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMRTIKVGSRRSKLAMTQTKWVIQKLKEINPSFAFEIKEIVTKGDRIVDVTLSKVGGKGLFVKEIEQALLNEEIDMAVHSMKDMPAVLPEGLVIGCIPEREDPRDALISKNRVKLSEMKKGAVIGTSSLRRSAQLLIERPDLTIKWIRGNIDTRLQKLETEDYDAIILAAAGLSRMGWKQDVVTEFLEPERCLPAVGQGALAIECRESDEELLALFSQFTDEYTKRTVLAERAFLNAMEGGCQVPIAGYS-VLNGQDEIEMTGLVASPDGKIIFKETVTG--NDPEEVGKRCAALMADKGAKDLIDRVKRELDEDGK 1YPN Chain:A ((5-306)) ---VLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTRGDV----------GQGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICEREDPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLDNGEYDAIILAVAGLKRLGLESR-IRAALPPEISLPAVGQGAVGIECRLDDSRTRELLAALNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDG--EIWLRALVGAPDGSQIIRGERRGAPQDAEQMGISLAEELLNNGAREILAEV---------

General information: TITO was launched using: RESULT: Template: 1YPN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1604 -85670 -53.41 -296.44 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.82 3D Compatibility (PKB) : -53.41 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.585

(partial model without unconserved sides chains): PDB file : Tito_1YPN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YPN-query.scw PDB file : Tito_Scwrl_1YPN.pdb: