Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVRKTNFR-DDCLYVGITKKGKAHLYCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVD-QASPFHCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTPTGSTAYNKSVAGAVVDPLLPCMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIEIKLSNKKIKTVKLKDNSFWEKVKRTFL 2I2D Chain:A ((35-262)) -----------------------------------------EPEIVISIGGDGTFLSAFHQYEERLDEIAFIGI-HT---GFYADWRPAEADKLVKLLAKGEYQKVSYPLLKTTVKY-EATYLALNESTVKSSG-GPFVVDVVINDIHFERFRGDGLCMSTPSGTTAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQ--ISVDHLSILHRDVQEIRYEVSAKKIHFARFRSFPFWRRVHDSFI

General information: TITO was launched using: RESULT: Template: 2I2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1170 -7702 -6.58 -35.33 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -6.58 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_2I2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I2D-query.scw PDB file : Tito_Scwrl_2I2D.pdb: