Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKILVTGAAGFIGSHLCEELLKDKKHNVIGIDDFIGPTPFSLKLKNLKNLLPEKRFTFIKENLLTADLASLLEGVDVIFHLAAIPGVRSSWGNHFHPYAAHNIQALQRLLEACREHSIQTFVFASTSSVYGEKQ-GKVSEN-----TSLSPLSPYGVTKLTGEKLCHVYKQSFGIPIVILRFFTVYGPRQRPD--MAFHRLIKQHLQQKPLTIFGDGQQSRDFTYISDCVKGITAVLGKPHLIGETVNIGGAERASVLKVVSLIEDISGRKATLHFSDKIAGEPSNTWADISKAKQLLHYDPATSLKDGLTNEIAYLSSLYQGE 2B69 Chain:A ((29-330)) -RILITGGAGFVGSHLTDKLMMDG-HEVTVVDNF-----FTGRKRNVEHWIGHENFELINHDVVEP----LYIEVDQIYHLASPASPPNYMYNPIKTLKTNTIGTLNMLGLAKRVGA--RLLLASTSEVYGDPEVHPQSEDYWGHVNPIGPRACYDEGKRVAETMCYAYMKQEGVEVRVARIFNTFGPRMHMNDGRVVSNFILQALQGEPLTVYGSGSQTRAFQYVSDLVNGLVALMNSN--VSSPVNLGNPEEHTILEFAQLIKNLVGSGSEIQFLSEAQDDPQKRKPDIKKAKLMLGWEPVVPLEEGLNKAIHYF-------

General information: TITO was launched using: RESULT: Template: 2B69.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1639 -55019 -33.57 -187.14 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -33.57 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.462

(partial model without unconserved sides chains): PDB file : Tito_2B69.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2B69-query.scw PDB file : Tito_Scwrl_2B69.pdb: