Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLWELFVAAILGIVEGLTEYAPVSSTGHMIIVDDIWLKSSNLMSEEAANSFKVVIQLGSILAVAIVFKDRILNLLGLKKNITSDQEQGHKLSIAQIAVGLVPAAVLGFLFEDYIDEYLFSVKTVAIGLIAGAILMLFADWVNKRKTATDTLDRISYKQAIAVGLFQCLSLWPGFSRSGSTISGGVILGLNHRAAADFTFIMAMPIMMGASFLSLVKHWDSLSSDLMPFFIVGFICAFVVALFVVRFFLRLINKIKLVPFAIYRIILGVILLLIMM 2Y1W Chain:A ((285-319)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------RCLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIV---------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y1W.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 44 -10634 -241.68 -303.83 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : -241.68 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.620

(partial model without unconserved sides chains): PDB file : Tito_2Y1W.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y1W-query.scw PDB file : Tito_Scwrl_2Y1W.pdb: