Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHIYKKLGAAFFALLLIAALAACGNNSESKGSASDSKGAETFTYKAENGNVKI--PKHPKRVVVMADGYYGYFKTLGINVVGAPENVFKNPYYKGKTNGVENIGDGTSVEKVIDLNPDLIIVWTTQGADIKKLEKIAPTVAVKYDKLDNIEQLKEFAKMTGTEDKAEKWLAKWDKKVAAAKTKIKKAVGDKT-ISIMQTNGKDIYVFGKDFGRGGSIIYKDLGLQATKLTKEKAIDQGPGYTSISLEKLPDFAGDYIFAGPWQSGGDDGGVFESSIWKNLNAVKNGHVYKMDPIGFYFTDPISLEGQLEFITESLTK 5FLY Chain:A ((3-266)) -------------------------------------KETKAFNLKTAKGEEKIDIPKDPKRIVVMAPTYAGGLKYLDANIVGVSDQVDQSPVLAKQFKDVDKVG-AEDVEKVASLKPDLIITYNT-DKNTDKLKKIAPTIAFDYAKYNYLEQQEAMGDIVGKSDEVKKWKADWEKQTAQDSKDIKAHLGDDTSVTIFEDFDKKIYAYGKNWGRGSEVLYQAFGLQMPKALDDAT--KKEGWTEVPKEEVGKYAGDVIITAKAKDAA-QPEFQKTAMWQNLEAVQNKYAFNVDSSVYWYNDPYTLD------------

General information: TITO was launched using: RESULT: Template: 5FLY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1317 81324 61.75 311.58 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : 61.75 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.539

(partial model without unconserved sides chains): PDB file : Tito_5FLY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FLY-query.scw PDB file : Tito_Scwrl_5FLY.pdb: