Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQLIQAQKKLLPDLLLVMQKRFEILQYIRLTEPIGRRSLSASLGISERVLRGEVQFLKEQNLVDIKTNGMTLTEEGYELLSVLEDTMKDVLGLTLLEKTLKERLNLKDAIIVSGDSDQSPWVKKEMGRAAVACMKKRF-SGKNIVAVTGGTTIEAVAEMM-TPDSKNRELLFVPARGGLGEDVKNQANTICAHMAEKASGTYRLLFVPGQLSQGAYSSIIEEPSVKEVLNTIKSASMLVHGIGEAKTMAQRRNTPLEDLKKIDDNDAVTEAFGYYFNADGEVVHKVHSVGMQLDDIDAIPDIIAVAGGSSKAEAIEAYFKK-PRNTVLVTDEGAAKKLLRDE 2O0M Chain:A ((95-341)) ----------------------------------------------------------------------------------------------HQIEKEMTQYFGIQRCIVVAGDSDIQKKVLSDFGDVLTNTLNLLLPNGENTIAVMGGTTMAMVAENMGSLETEKRHNLFVPARGGIGEAVSVQANSISAVMANKTGGNYRALYVPEQLSRETYNSLLQEPSIQEVLTLISHANCVVHSIGRALHMAARRKMSDDEMVMLKQKNAVAESFGYFFDEEGKVVYKIPRIGLQLKNLQEIPYVVAIAGGKTKAKAIRAYMKNAPKQTWLITDEAAANEILK--

General information: TITO was launched using: RESULT: Template: 2O0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1388 19078 13.74 78.19 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 13.74 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.604

(partial model without unconserved sides chains): PDB file : Tito_2O0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O0M-query.scw PDB file : Tito_Scwrl_2O0M.pdb: