Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKNGELKPGDKLDSVQALAESFQVSRSAVREALSALKAMGLVEMKQGEGTYLKEFELNQISQPLSAALLMKKEDVKQLLEVRKLLEIGVASLAAEKRTEADLERIQDALK---EMGSIEADGELGEKADFAFHLALADASQNELLKHLMNHVSSLLLETMRETRKIWLFSKKTSVQRLY-----EEHERIYNAVAAGNGAQAEAAMLAHLTNVE-DVLSGYFEENVQ 2HS5 Chain:A ((41-232)) -IIDGTFRPGARL-SEPDICAALDVSRNTVREAFQILIEDRLVAHELNRGVFVR---------------VPTAEDITELYICRRVVE--CAGVNGFDPATGDLSRVAEALDLADERYAVEDWTGVG-TADIHFHSALASLNNS-------NRIDELMRSVWNEARLV--FHVMDDAHRFHGPYLTRNHE-IYDALAAGNTEAAGQLLKTYLEDAEAQILGAY------

General information: TITO was launched using: RESULT: Template: 2HS5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 701 -27959 -39.88 -152.78 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -39.88 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.533

(partial model without unconserved sides chains): PDB file : Tito_2HS5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HS5-query.scw PDB file : Tito_Scwrl_2HS5.pdb: