Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAIQNPYTAYQQNSVNTATPGELTLMLYNGCLKFIRLAAQAIEN-DDMERKN---ENLIKAQNIIQELNFTLN--RNIELSASMGAMYDYMYRRLVQAN-IKNDTGMLAEVEGYVTDFRDAWKQAIQSERKDRHGSGGIA 1ORJ Chain:A ((1002-1119)) ---RNIAEAYFQNMVETATPLEQIILLYDKAIECLERAIEIYDQVNELEKRKEFVENIDRVYDIISALKSFLDHEKGKEIAKNLDTIYTIILNTLVKVDKTKEELQKILEI---LKDLREAWEE----------------

General information: TITO was launched using: RESULT: Template: 1ORJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 321 -20412 -63.59 -183.89 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -63.59 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_1ORJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ORJ-query.scw PDB file : Tito_Scwrl_1ORJ.pdb: