Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVITYGTFDLLHWGHIKLLERAKQL--GDYLVVAISTDE-------FNLQKQKKAYHSYEHRKLILETIRYVDEVIPEKNWEQKKQDIIDHNIDVFVMGDD----WEGKFDFLKDQCEV-VYLP--RTEGISTTKIKEEIAGL 3HL4 Chain:A ((78-205)) --RVYADGIFDLFHSGHARALMQAKNLFPNTYLIVGVCSDELTHNFKGFTVMNENERYDAVQH-------CRYVDEVVRNAPWTLTPEFLAEHRID-FVAHDDIPYSSAGSDDVYKHIKEAGMFAPTQRTEGISTSDI-------

General information: TITO was launched using: RESULT: Template: 3HL4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 480 -7116 -14.82 -63.53 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -14.82 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.541

(partial model without unconserved sides chains): PDB file : Tito_3HL4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3HL4-query.scw PDB file : Tito_Scwrl_3HL4.pdb: