Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNITVIGTGYVGLVTGVSLSEIGHHVTCIDIDAHKIDEMRKGISPIFEPGLEELMRKNTADGRLNFETSYEKGLAQADIIFIAVGTPQKSDGHANLEQITDAAKRIAKHVK---RDTVVVTKSTVPVGTNDLINGLITEHLAEP---VSISVASNPEFLREGSAIYDTFHGDRIVIGTADEKTANTLEELFRPFQIPIYQTDIRSAEMIKYASNAFLATKISFINEISNICEKVGADIEAVAYGMGQDKRIGSQFLKAGIGYGGSCFPKDTNALVQIAGNVEHDFELLKSVIKVNNNQQAMLVDKALNRLGGVTGKTIALLGLSFKPNTDDMREAPSIVIADRLAALDARIRAYDPIAVSHAKHVLPQAVEYKETIEEAVKGSDAVMILTDWADIKQFPLAAYQDL-METPLIFDGRNCYTLDEALAAGVEYYSVGRKAVVPSGAIQ 3VTF Chain:A ((23-435)) -SLSVLGLGYVGVVHAVGFALLGHRVVGYDVNPSIVERLRAGRPHIYEPGLEEALGRALSSGRLSFAESAEEAVAATDATFIAVGTPPAPDGSADLRYVEAAARAVGRGIRAKGRWHLVVVKSTVPPGTTE---GLVARAVAEEAGGVKFSVASNPEFLREGSALEDFFKPDRIVIGAGDERAASFLLDVYKAVDAPKLVMKPREAELVKYASNVFLALKISFANEVGLLAKRLGVDTYRVFEAVGLDKRIGRHYFGAGLGFGGSCFPKDTLAFIRFGESLGLEMAISKAVLRVNEYMPRYAVQLLEERLGGLRGRHVGVLGLAFKPNTDDVRESRGVEVARLLLERGARVYVHDPMAMEKARAVLGDSVTYVEDPQALLDQVEGVIIATAWPQ--------YEGLDYRGKVVVDGRYVKKAREA----------------------

General information: TITO was launched using: RESULT: Template: 3VTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2318 -25088 -10.82 -61.79 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -10.82 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.580

(partial model without unconserved sides chains): PDB file : Tito_3VTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3VTF-query.scw PDB file : Tito_Scwrl_3VTF.pdb: