Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTFKLVDLNVERVDKEEQSIEQFPLIDGLIINKEDGENHWLIEALVQKEHLSFFEQLQNSQSEAKVFVTITKKSNRPAQLTAAVKNIVKLEESIQVLLYGQMVTRKQQGTETILES---LVKE-GYTGTKLIEAFKQKL 4ZEB Chain:A ((253-339)) ---------------------------------------------LIENFHMFTFNMNQEVFKDKKLRRALALAVNRPIMVEALWKKQASIPAGFNFPNYGETFDPKRKAMEYNVEEAKRLVKESGYDGTPI--------

General information: TITO was launched using: RESULT: Template: 4ZEB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 199 -976 -4.90 -11.75 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -4.90 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.302

(partial model without unconserved sides chains): PDB file : Tito_4ZEB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZEB-query.scw PDB file : Tito_Scwrl_4ZEB.pdb: