Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFNRLFRVCFLAALIMAFTLPNSVYAQKPIFKEVSVHDPSIIETNGTFYVFGSHLASAKSNDLMQWQQLTTSVSNDNPLIPNVYEELKETFEWAQS-DTLWAADVTQLADGKYYMYYNACRGDSPRSAMGVAVADNIEGPYKNKGIFLKSG----MEGTSSDGTPYDATKHPNVVDPHTFFDKDGKLWMVYGSYSGGIFILEMNPKTGFPLPGQGYGKKLLGGNHSRIEGPYVLYNPDTQYYYLYLSYGGLDATGGYNIRVARSKKPDGPYYDAEGNPMLDVRGKGGTFFDDRSIEPYGVKLMGSYTFETENE---KGTGYVSPGHNSAYYDEKTGRSYLIFHTRFPGRGEEHEVRVHQLFMNKDGWPVAAPYRYAGETLKEVKQKDITGTYKLIQHGKDISADIKQTINIQLNKNHTISGEMTGTWRKTGKNTADITLAGKK---YNGVFLRQWDSVREKNVMTFSVLNTSGEAVWGSK 4KC7 Chain:A ((25-471)) ---------------------------QPTFRWAVVHDPSIIKVGNMYYVFGTHLQVAKSKDLMHWEQINTSAHDKNPIIPNINEELKETLSWARTRNDIWAPQVIQLSDGRYYMYYCASTFGSPRSAIGIAVSDDIEGPYKHYAVIVKSGQVYSVDGPSEDGTPYDSRKHPNALDPGVFYDKEGNLWMVYGSWFGGIYILKLDPNTGLPLPGQGYGKRLVGGNHSSMEGPYILYSPDTDYYYLFLSFGGLDYRGGYNIRVARSKNPNGPYYDPEGKSMENCMG------SKTVISNYGAKLVGNFILSESNTIDFKAFGYVSPGHNSAYYDPETGKYFIFFHTRFPGRGETYQLRVHQLFLNEDGWFVMAPFPYGGETVSKLPNEEIVGEYQFINHGKEITDKIKQPVRIKLNSDGSITGAVEGRWERKEHYITLKIIEGNTTVIYKGVLLKQWHYSEKKWVTVFTALSNQGVSVWGIR

General information: TITO was launched using: RESULT: Template: 4KC7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2800 62084 22.17 142.39 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.84 3D Compatibility (PKB) : 22.17 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.649

(partial model without unconserved sides chains): PDB file : Tito_4KC7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4KC7-query.scw PDB file : Tito_Scwrl_4KC7.pdb: