Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSEKKNIYPNKEGCPVEFTLDVIGGKWKGILFYHMIDGKKRFNEFRRICPSITQRMLTLQLRELEADGIVHREVYHQVPPKVEYSLTEFGRTLEPIVLQMKEWGESNRDVLESYRSNGLVKDQQK 2F2E Chain:A ((9-101)) ----------QASCPVARPLDVIGDGWSMLIVRDAFEGLTRFGEFQKSL-GLAKNILAARLRNLVEHG-VMVAVPAESGSHQEYRLTDKGRALFPLLVAIRQWGE--------------------

General information: TITO was launched using: RESULT: Template: 2F2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 286 -40307 -140.93 -433.41 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -140.93 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.406

(partial model without unconserved sides chains): PDB file : Tito_2F2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F2E-query.scw PDB file : Tito_Scwrl_2F2E.pdb: