Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLRTEKEKMAAGELYNSEDQQLLLERKHARQLIRQYNETPEDD-AVRTKLLKELLGSVGDQVTILPTFRCDYGYHIHIGDHTFVNFDCVILDVCEVRIGCHCLIAPGVHIYTAGHPLDPIERKSGKEFGKPVTIGDQVWIGGRAVINPGVTIGDNAVIASGSVVTKDVPANTVVGGNPARILKQL 3SRT Chain:A ((5-186)) ---TEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEDEYEKREDILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVMLAPNVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGITIGDNVVIGAGSVVTKDIPPNTVAVGNPCRVIKKI

General information: TITO was launched using: RESULT: Template: 3SRT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 933 -42431 -45.48 -234.42 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.87 3D Compatibility (PKB) : -45.48 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.612

(partial model without unconserved sides chains): PDB file : Tito_3SRT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SRT-query.scw PDB file : Tito_Scwrl_3SRT.pdb: