Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLNSANTTAPSLLSAYGLNSYTSSNSGSVTKAAESTETAVADSASNKHEANQIRSGDFSIDSAIKKAADKYGVDEKLIRAVIKQESGFNAKAVSGAGAMGLMQLMPSTA-----SSLGV------SNPLDPQQNVEGGTKYLKQMLDK----YDG--NVSMA-LAAYNAGPGNVDRYGG---------I---------------PPFQETQNYVKKITSVYYA 4HJZ Chain:A ((34-204)) ---------------------------------------------------VPVQRAMQWMPISQKAGAAWGVDPQLITAIIAIQSGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEPTTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSDRKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALD-

General information: TITO was launched using: RESULT: Template: 4HJZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 561 -47513 -84.69 -368.31 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -84.69 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.476

(partial model without unconserved sides chains): PDB file : Tito_4HJZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HJZ-query.scw PDB file : Tito_Scwrl_4HJZ.pdb: