Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAKTKLVTDRIRLRCMEDRDQATLFGLLFNDPDVMTYYSGLKDKRQTREWVNWNQRNEKGYGVSLWIAEDKRTGEFLGQCGIVPQQIENQTVMEIGYMFARRHWGNGYAQEAARACLDYGFNERQFGKMAALIDPGNKASIRVAEKIGMHYSKTI--------RKWNKPIAVYERKSYN---- 4YGO Chain:D ((2-173)) --------NSQLTLRALE-RGDLRFIHNLNNNRNIMSYWFE-EPYESFDELEELYNKHIHDNAERRFVVEDAQ-KNLIGLVELIEINYIHR-SAEFQIIIAPEHQGKGFARTLINRALDYSFTILNLHKIYLHVAVENPKAVHLYEECGFVEEGHLVEEFFINGRYQDVKRMYILQSKYLNRSE

General information: TITO was launched using: RESULT: Template: 4YGO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 662 -87679 -132.45 -547.99 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : -132.45 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.411

(partial model without unconserved sides chains): PDB file : Tito_4YGO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YGO-query.scw PDB file : Tito_Scwrl_4YGO.pdb: