Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNEKRLLEEFLELVQIDSETKHEAEICKVLKRKFSDLGVDVKEDDTMDITGHGAGNLICTLKGTKQTDTIYFTSHMDTVVPGNG-VKPVV--ENGYVKTDGTTILGADDKAGLAAMFEAIKVLKEENIEHGTIEFIITVGEESGLIGAKALDRSM-ITASYGYALDSDG---KVGNIIVAA----PTQAKVRAAIFGKTAHAGVEPEKGISAITIASKAISKMPLGRIDEETTANIGRFEGGTQTNIVCDEVHILAEARSLVPEKMEAQVQKMKAAFEEAAADMGGRAEVEIEVMYPGFKYQDGDQVVEIAKKAAAKIGRPS-ELQTSGGGSDANVIAGHGIPT--VNLAVGY-EQIHTKNEKMPIEELVKTAEMVVAIIEEAAK 1YSJ Chain:A ((30-400)) --FHTRLINMRRDLHEHPELSFQEVETTKKIRRWLEEEQIEILDVP------QLKTGVIAEIKGREDGPVIAIRADIDALPIQEQTNLPFASKVDGTMHACGH----DFHTASIIGTAMLLNQRRA--ELKGTVRFIFQPAEEIA-AGARKVLEAGVLNGVSAIFGMHNKPDLPVGTIGVKEGPLMASVDRFEIVIKGK---------NSIDPIAAAGQIISG------LQNAVVSITRVQAGTSWNVIPDQAEMEGTVRTFQKEARQAVPEHMRRVAEGIAAGYGAQAEFKWFPYLPSV--QNDGTFLNAASEAAARLGYQTVHAEQSPGGEDFALYQE-KIPGFFVWMGTNGTEEWHHPAFTLDEEALTVASQYFAELAVIVL-

General information: TITO was launched using: RESULT: Template: 1YSJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1832 17481 9.54 51.87 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 9.54 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_1YSJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YSJ-query.scw PDB file : Tito_Scwrl_1YSJ.pdb: