Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFAVIVLPGSNCDIDMYHAVKDELGHEVEYVWHEETSLDGFDGVLIPGGFSYGDYLRCGAIARFANIMPAVKQAAAEGKPVLGVCNGFQILQELGLLPGAMRRN-KDL---K------------------------FICRPV-ELIVQNDETLFTASYEKGESITIPVAHGEGNFYCDDETLATLKENNQIAFTYGSNINGSVSDIAGVVNEKGN-VLGMMPHPERAVDELLGSADGLKLFQSIVKNWRETHVTTA 2V4U Chain:A ((361-552)) --------------------------------------LCKADGILVPGGFGIRGT---------LGKLQAISWARTKKIPFLGV-LGMQLAVIEF---ARNCLNLKDADSTEFRPNAPVPLVIDMPEHNPGNLGGTMRLGIRRTVFKT-ENSILRKLYG--DVPFIEERHRHR-FEVNPNLIKQFEQNDLSFVGQ--DVDGD--RMEIIELANHPYFVGVQFHPEFSSRPMKP----SPPYLGLLLAATGNLN---

General information: TITO was launched using: RESULT: Template: 2V4U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 764 -64670 -84.65 -401.68 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -84.65 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.457

(partial model without unconserved sides chains): PDB file : Tito_2V4U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2V4U-query.scw PDB file : Tito_Scwrl_2V4U.pdb: