Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIKDLMTADLQYCTVLDNVYEAAVKMKD-----ANVGAIPVVDEDGETLVGIVTDRDLVLRGIAIKKPNSQKITDAMTEKPVSVEEDASVDEVLHLMASHQLRRIPVTK-NKKLTGIVTLGDLSLSEQTNERAGSALSDISEGDNREEGFFH 2OUX Chain:A ((138-262)) --TAGAIMTTEFVSIVANQTVRSAMYVLKNQADMAETIYYVYVVDQ-ENHLVGVISLRDLIVND------DDTLIADILNERVISVHVGDDQEDVAQTIRDYDFLAVPVTDYDDHLLGIVTVDDIIDVIDDEAA--------------------

General information: TITO was launched using: RESULT: Template: 2OUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 534 -89386 -167.39 -751.14 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -167.39 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_2OUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OUX-query.scw PDB file : Tito_Scwrl_2OUX.pdb: