Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLPLHISLEKKKVVIAGGGSIALRRLKTVISEGADITLVSPDVEPEIKQMAEERRIKWEKRTIE------KEDYLNAFFIIAATDNAAVNKEIAQSASP--FQL--VNCVSDAELGNVYMPKIVKRGHVTVSVSTSGASPKHTKELAENVDKLIDGDFVAEVNRLYQMRRKK 3L4B Chain:C ((1-107)) ----------MKVIIIGGETTAYYLARSMLSRKYGVVIINKDRE-LCEEFAKKLKATIIHGDGSHKEILRDAEVSKNDVVVILTPRDEVNLFIAQLVMKDFGVKRVVSLVNDPGNMEI------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3L4B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 439 -59607 -135.78 -614.51 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : -135.78 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_3L4B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3L4B-query.scw PDB file : Tito_Scwrl_3L4B.pdb: