Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKVLLGFAAFTLSLSLAACSSNDSEKVSTEKETPQASTDVEKKTEQKESTKEKTADKSKEKDKKELVDVTLDRAVDGDTIKVTYNGNVDTVRYLLIDTPETKKPNSCVQPYGEDASKRNKELVNSGK-LQLEFDKGDRRDKYGRLLAYVYVDGKSVQETLLKEGLARVAYVYEPNTKYIDQFKKDEQEAKSEKLSIWSKNGYVTDRGFNGCVKEKTTAVKKATTSKPAATQPTTPKASSETSTTTEKEASSETTGGTETFKNCTELRKKYPNGVPSSHPAYQSKMDRDHDNYACER 3T13 Chain:B ((13-135)) ----------------------------------------------------------------------TLIKAIDGDTVKLMYKGQPMTFRLLLVDTPEFN------EKYGPEASAFTKKMVENGKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKGNNTHEQLLRKAEAQAKKEKLNIWS--------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3T13.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 602 11699 19.43 95.89 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : 19.43 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3T13.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T13-query.scw PDB file : Tito_Scwrl_3T13.pdb: