Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNVLTIKGVSIGEGMPKIIIPLMGKTEKQILNEAEAVKLLNPDIVEWRVDVFEKANDREAVTKLISKLRKSLEDKLFLFTFRTHKEGGSMEMDESSYLALLESAIQTKDIDLIDIELFSGDANV---KALVSLAEENNVYVVMSNHDFEKTPVKDEIISRLRKMQDLGAHIPKMAVMPNDTGDLLTLLDATYTMKTIYADRPIITMSMAATGLISRLSGEVFGSACTFGAGEEASAPGQIPVSELRSVLDILHKNTRG 1SFJ Chain:B ((32-233)) ------------------------------------------DVLELRIDQFENVT-VDQVAEMITKL---QDSFKLLVTYRTKLQGGYGQFTNDSYLNLISDLANINGIDMIDIE-WQADIDIEKHQRIITHLQQYNKEVIISHHNFESTPPLDELQFIFFKMQKFNPEYVKLAVMPHNKNDVLNLLQAMSTFSDT-MDCKVVGISMSKLGLISRTAQGVFGGALTYGCIGEPQAPGQIDVTDLKA-----------

General information: TITO was launched using: RESULT: Template: 1SFJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1006 -64040 -63.66 -326.73 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : -63.66 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_1SFJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SFJ-query.scw PDB file : Tito_Scwrl_1SFJ.pdb: