Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKLYGVIGNPIGHSMSPDIHNASL----KDLGLDGHYHAFKVEENDLEDAVKG-IRALGVQGINVTVPHKVSIMDYLDHIDESAKVLGAVNTVRREGDKLVGYNTDGEGFVKSLMKVLDKPISELSFLMIGAGGAARAIFTTIVRNTPKKFDICNRT---LEKAKRLTEATPSFHNKEVLSIKEAEERLEQYDVIIHTTSVGMYPNVDDVPLSLQ----RAASSAVVCDIVYNPIQTALLKEASQKGLKTLDGVGMFVEQAALSFQLWTGQE----PNIEKMRSIVIGKLGGTEC 3PHJ Chain:A ((4-263)) --KSFGVFGNPIKHSKSPLIHNACFLTFQKELRFLGHYHPILL---PLESHIKSEFLHLGLSGANVTLPFKERAFQVCDKIKGIALECGAVNTLVLENDELVGYNTDALGFYLSL-----K---YQNALILGAGGSAKAL-ACELKKQGLQVSVLNRSSRGLDFFQRL--GCDCF----------MEPPKSAFDLIINATS--------ELPLNKEVLKGYFKEGKLAYDLAYGFL-TPFLSLAKELKTPFQDGKDMLIYQAALSFEKFSASQIPYSKAFEVMRSVF---------

General information: TITO was launched using: RESULT: Template: 3PHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1359 45298 33.33 191.94 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : 33.33 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.483

(partial model without unconserved sides chains): PDB file : Tito_3PHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3PHJ-query.scw PDB file : Tito_Scwrl_3PHJ.pdb: