Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGNTRKKVSVIGAGFTGATTAFLIAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTQEIVKYSPDSIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLI-----PKERIDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEMVEAILKDQRRVLPTIAYLEGEYGYEGIYLGVPTIVGGNGLEQIIELELTDYERAQLNKSVESVKNVMKVLS 3GVH Chain:C ((6-316)) ---ARNKIALIGSGMIGGTLAHLAGLKELGDVVLFDIA--EGTPQGKGLDIAESSPVDGFDAKFTGANDYAAIEGADVVIVTAGVP-----SRDDLLGINLKVMEQVGAGIKKYAPEAFVICITNPLDAMVWALQKFSGLPAHKVVGMAGVLDSARFRYFLSEEFNVSVEDVTVFVLGGHGDSMVPLARYSTVAGIPLPDLVKMGWTSQDKLDKIIQRTRDGGAEIVGLLKTGSAFYAPAASAIQMAESYLKDKKRVLPVAAQLSGQYGVKDMYVGVPTVIGANGVERIIEIDLDKDEKAQFDKSVASVAGLCEAC-

General information: TITO was launched using: RESULT: Template: 3GVH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1663 -124766 -75.02 -414.50 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain C : 0.88 3D Compatibility (PKB) : -75.02 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_3GVH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GVH-query.scw PDB file : Tito_Scwrl_3GVH.pdb: