Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKIESTQELEKAV-KDDWSVFMFSADWCPDCRFVEPFLPELEANFPEFTYYYVDRDKFIDTCAEWEIYGIPSFVVFNEGKEVNRFVSKDRKTKEEIEQFLTDSLAKA 4RUV Chain:A ((2-105)) -QSIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVEQYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLAETF---

General information: TITO was launched using: RESULT: Template: 4RUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 440 -9144 -20.78 -88.78 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -20.78 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.614

(partial model without unconserved sides chains): PDB file : Tito_4RUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RUV-query.scw PDB file : Tito_Scwrl_4RUV.pdb: