Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYIMKQSGWLELICGSMFSGKSEELIRRVKRATYAKQEVRVFKPVIDNRYSEAAVVSHNGTSMTSYAISSAADIWDHISESTDVVAVDEVQFFDQEIVEVLSSLADKGYRVIAAGLDMDFRGEPFGVVPNIMAIAESVTKLQAVCSVCGSPASRTQRLIDGKPASYDDPVILVGAAESYEARCRHHHEVPGKSKK 2J87 Chain:D ((4-171)) -------GHIQLIIGPMFSGKSTELIRRVRRYQIAQYKCVTIKYSNDNRYG-T--WTHDKNNFEALEATKLCDVLQSITDFS-VIGIDEGQFFP-DIVEFCERMANEGKIVIVAALDGTFQRKPFNNILNLLILSEMVVKLTAVCMKCFKEASFSKRL------GEETEIEIIGGNDMYQSVCR-----------

General information: TITO was launched using: RESULT: Template: 2J87.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 840 -107726 -128.25 -648.95 target 2D structure prediction score : 0.49 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : -128.25 2D Compatibility (Sec. Struct. Predict.) : 0.49 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.330

(partial model without unconserved sides chains): PDB file : Tito_2J87.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J87-query.scw PDB file : Tito_Scwrl_2J87.pdb: