Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MNEGYIIAGLLLLTAGMIDFLWTTLWLESGAGPITRCLSAWLWKGCRKISGDHAKVLSMAGPLLLCLTLVIWISLFWSGWVLIYSSDPHSLMETQSKEPASWSDRIYFSGYVMFTLGNGDLAPNGGLWKLVT------IIETAQGLLTITFSVTYLISVLSAVNQKRSFAQSVLSLGHDGTEIVHNAWNGKDFHDIDFLLVAASSELGKLTAQHNAFPILHFYHSTQHQESSIIAVAVLDEALTIFKYGIPEQYQPNQLHIKEARSSIKNYLDTVHTAYIHPAEQAPPEPDISKLQQSGIPALSKQTFQIAVNSIKERRQLLLGIIQAGARKWPVQEQAIGNAYSPK 2P67 Chain:A ((2-329)) SLINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLLDAIMPYCG-NTLRLGVTGTPG------AGKSTFLEAFGMLLIREGLKVAVIAVDPS---NDLARAEAAFIRPVPSGASQRARELMLLCEAAGYDVVIVETVGVGQSETEVARMVDCFISLQIAQGIKKGLMEVA-------------------DLIVINKDDGDNHTNVAIARHMYESALHILRRKYDE-------------WQPRVLTCSALEKRGIDEIWHAIIDFKTALTAS---GRLQQVRQQQSVEWLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQT--

General information: TITO was launched using: RESULT: Template: 2P67.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1235 -142151 -115.10 -483.51 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -115.10 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_2P67.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P67-query.scw PDB file : Tito_Scwrl_2P67.pdb: