Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFAVIVLPGSNCDIDMYHAVKDELGHEVEYVWHEETSLDGFDGVLIPGGFSYGDYLRCGAIARFANIMPAVKQAAAEGKPVLGVCNGFQILQELGLLPGA-------------MR-RNKD---LKFICRPVELIVQNDETLFTASYEKGESITIPVAHGEGNFYCDDETLATLKENNQIAFTYGSNINGSVSDIAGVVNEKGNVLGMMPHPERAVDELLGSADGLKLFQSIVKNWRETHVTTA 4GUD Chain:A ((3-203)) QNVVIIDTGCA-NISSVKFAIERL-GYAVTISRDP-QVVLAADKLFLPGVGTASEA-----MKN-LTERDLIELVKRVEKPLLGICLGMQLLGKLS---EEKDEIVQCLGLVDGEVRLLQTGDLPLPHMGWNTVQVK-EGHPLFNGIEP--DAYFYFVHSFA---MPV---------GDYTIAQ--CEYGQ-PFSAAIQ--AGNYYGVQFHPERSSK--AG----ARLIQNFLEL---------

General information: TITO was launched using: RESULT: Template: 4GUD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 975 -131257 -134.62 -729.21 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -134.62 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_4GUD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GUD-query.scw PDB file : Tito_Scwrl_4GUD.pdb: