Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFHISTLDQIKIAYIGGGSQGWARSLMSDLSIDERMSGTVALYDLDFEAAQKNEVIGNHSGNGR-WRYEAVSTLKKALSAADIVIISILPGSLDDMEVDVHLPERCGIYQSVGDTVGPGGIIRGLRAVPIFAEIARAIRDYAPESWVINYTNPMSVCTRVLYKVFPGIKAIGCCHEVFGTQKLLAEMVTERLGIEVP---RREDIRVNVLGINHFTWITKASYRH-IDLLPIFREFSAHYGESGYELEGECWRDSVFCSAHRVAFDLFETYGAIPAAGDRHLAEFLPGPYLKQ-PEVWKFHLT-----PISFRKQDRAEKRQ-ETERLIVQQRGVAEKASGEEGVNIIAALLGLGELVTNVNMPNQGQVLNLPIQAIVETNAFITRNRVQPILSGALPKGVEMLAARHISNQEAVADAGLTKDTGLAFQAFLNDPLVQIDRSDAEQLFNDMLQCIMQS 3FEF Chain:A ((3-436)) ------LDQIKIAYIGGGSQGWARSLMSDLSIDERMSGTVALYDLDFEAAQKNEVIGNHSG-NGRWRYEAVSTLKKALSAADIVIISILPGSLDDMEVDVHLPERCGIYQSVGDTVGPGGIIRGLRAVPIFAEIARAIRDYAPESWVINYTNPMSVCTRVLYKVFPGIKAIGCCHEVFGTQKLLAEMVTERL--GIEVPRR-EDIRVNVLGINHFTWITKASY-RHIDLLPIFREFSAHYGESGYELEGECWRDSVFCSAHRVAFDLFETYGAIPAAGDRHLAEFLP-GPYLKQPEVWKFHLTPISFRK-QDRAEKRQETERLIVQQ----R-GVAEKASGEEGVNIIAALLGLGELVTNVNMPNQGQVLNLPIQAIVETNAFITRNRVQPILSGALPKGVEMLAARHISNQEAVADAGLTKDTGLAFQAFLNDPLVQIDRSDAEQLFNDML------

General information: TITO was launched using: RESULT: Template: 3FEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2495 -271265 -108.72 -642.81 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.94 3D Compatibility (PKB) : -108.72 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.546

(partial model without unconserved sides chains): PDB file : Tito_3FEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FEF-query.scw PDB file : Tito_Scwrl_3FEF.pdb: