Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSVNLSYPIGEYKPRESISKEQKDKWIQVLEEVPAKLKQAVEVMTDSQLDTPYRDGGWTVRQVVHHLADSHMNSYIRFKLSLTEETPAIRPYDEKAWSELKDSKTADPSGSLALLQELHGRWTALLRTLTDQQFKRGFYHPDTKEIITLENALGLYVWHSHHHIAHITELSRRMGWS 2F22 Chain:A ((4-133)) ---------------------------TNGVLYAANMTNALAKEIPESKWDIQLIPELGTLRKLFIHIVRVRDVYRDGLKT----GSIK---FPG----R----LASDEHRLLDELERSMEELVFEFKQTTFNSI----KM--GENYLSIMELLGTVIQHEGIHQGQYYVALKQSGIN

General information: TITO was launched using: RESULT: Template: 2F22.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 496 -58949 -118.85 -453.45 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -118.85 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2F22.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2F22-query.scw PDB file : Tito_Scwrl_2F22.pdb: