Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAQLFNLESRLDELENEINTQYCELDTNLDALKSNRIELESQLEKFESSLTNRLQGSISNNCRNDLLNLGYTHSQVDCMSDEEVYAALDKIDEEIHNTDQDY-STGF---EDLEKQIIEMKRDYFIDRKERGSGNFDEAWEGEILDLEFEYTVLCLEKGLEPLNYIITWEG 4D41 Chain:G ((90-141)) ---------------------------------------------------------------------------QIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDLRGRFSETSREGFL---------------------------------------------

General information: TITO was launched using: RESULT: Template: 4D41.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 49 -1775 -36.22 -36.98 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain G : 0.64 3D Compatibility (PKB) : -36.22 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.502

(partial model without unconserved sides chains): PDB file : Tito_4D41.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D41-query.scw PDB file : Tito_Scwrl_4D41.pdb: