Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNHLQLKKLLNHFFLEDIGTGDLTSQSIFGEQSCEAEIVAKSEGIFAGAAIIKEGFSLLDENVQSILHKKDGDMLHKGEVIAELHGPAAALLSGERVVLNLIQRLSGIATMTREAVRCLDDEQIK--ICDTRKTTPGLRMLEKYAVRAGGGYNHRFGLYDGIMIKDNHIAACGSILEACKKARQAAGHMVNIEVEIETEEQLREAIAAGADVIMFDNCPPDTVRHFAKLTPA---NIKTEASGGITLESLPAFKGTGVNYISLGFLTHSVKSLDISMDVTLSNESVEECCYVNS 5AYZ Chain:G ((10-285)) LPPVTLAALVDSWLREDC-PGLNYAALVSGAGPSQAALWAKSPGVLAGQPFFDAIFTQLNCQVSWFL--PEGSKLVPVARVAEVRGPAHCLLLGERVALNTLARCSGIASAAAAAVEAARGAGWTGHVAGTRKTTPGFRLVEKYGLLVGGAASHRYDLGGLVMVKDNHVVAAGGVEKAVRAARQAADFALKVEVECSSLQEAVQAAEAGADLVLLDNFKPEELHPTATVLKAQFPSVAVEASGGITLDNLPQFCGPHIDVISMGMLTQAAPALDFSLKL---------------

General information: TITO was launched using: RESULT: Template: 5AYZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 1394 -63469 -45.53 -234.20 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain G : 0.80 3D Compatibility (PKB) : -45.53 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_5AYZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AYZ-query.scw PDB file : Tito_Scwrl_5AYZ.pdb: