Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVAIASDHGGVHIRNEIKELMDELQIEYIDMGCDCGSGSVDYPDYAFPVAEKVVSGEVDRGILICGTGIGMSISANKVKGIRCALAHDTFSAKATREHNDTNILAMGERVIGPGLAREIAKIWLTTEFTGGRHQTRIGKISDYEEKNL 4LFM Chain:B ((1-128)) MIIAIGNDHIVTMQKIEISNMLKDMGYTVIDEGT-YDTHRTHYPIYGKKVAEDVADGRADLGIVMCGTGIGISTAADKNEGIRAAMCDDVTSAVYAREQLNANVLGIGGAVVGVHLIQDIVKAYLDATY--------------------

General information: TITO was launched using: RESULT: Template: 4LFM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 743 -96525 -129.91 -754.10 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -129.91 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_4LFM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LFM-query.scw PDB file : Tito_Scwrl_4LFM.pdb: