Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLIEREKTVYYKEKPDPSSLGFGQYFTDYMFVMDYEEGIGWHHPRIAPYAPLTLDPSSSVFHYGQAVFEGLKAYRTDDGRVLLFRPDQNIKRLNRSCERMSMPPLDEELVLEALTQLVELEKDWVPK-EKGTSLYIRPFVIATEPSLGVKASRSYTFMIVLSPVGSYYGDDQLKPVRIYVEDEYVRAVNGGVGFAKTAGNYAASLQAQRKANELGYDQVLWLDAIEKKYVEEVGSMNIFFVI-NGEAVTPALSGSILSGVTRASAIELI-RSWGIPVREERISIDEVYAASARGELTEVFGTGTAAVVTPVGELNIHGKT--VIVGDGQIGDLSKKLYETITDIQLGKVKGPFNWTVEV 3UZO Chain:B ((25-358)) -----------------DWSTLGFSYIRTDLRYLAHWKDG-EWDAGTLTEDNQIHLAEGSTALHYGQQCFEGLKAYRCADGSINLFRPDQNAARMRMSCRRLLMPELSDEQFIDACLQVVRANEHFLPPYGTGGSLYLRPFVIGVGDNIGVRTAPEFIFSVFCVPVGPYF-KGGLTPTN-FITSDYDRAAPHGTGAAKVGGNYAASLLPGYEAKKRDFADVIYLDPATHTTIEEAGAANFFAITQDGQKFVTPQSPSILPSITKYSLLWLAEHRLGLEVEEGDIRIDEL------GKFSEAGACGTAAVITPIGGIQ-HGDDFHVFYSESEPGPVTRRLYDELVGIQYGDKEAPEGWIVKV

General information: TITO was launched using: RESULT: Template: 3UZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1852 -186166 -100.52 -565.85 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -100.52 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_3UZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UZO-query.scw PDB file : Tito_Scwrl_3UZO.pdb: