Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKFAVIVLPGSNCDIDMYHAVKDELGHEVEYVWHEETSLDGFDGVLIPGGFSYGDYLRCGAIARFANIMPAVKQAAAEGKPVLGVCNGFQILQELGLLPGAMRR------NKDLKFICRPVELIVQNDETLFTASYEKGESITIPVAHGEGNF-YCDDETLATLKENNQIAFTYGSNINGSVSDIAGVVNEKGNVLGMMPHPERAVDELLGSADGLKLFQSIVKNWRETHVTTA 2YWJ Chain:A ((1-185)) MIIGVLAIQGDV--EEHEEAIKKA-GYEAKKVKRVE-DLEGIDALIIPGGESTAIG----KL---MKKYGLLEKIKNSNLPILGTCAGMVLLSKGT---GINQILLELMDITVKRNAYGR-QVDSF-EKEIEFKDLGK--VYGVF-IRAPVVDKIL---------SDDVEVIAR--DGD-K---IVGVKQ--GKYMALSFHPELSED---G----YKVYKYFVENCVK------

General information: TITO was launched using: RESULT: Template: 2YWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1022 -92900 -90.90 -521.91 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -90.90 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.344

(partial model without unconserved sides chains): PDB file : Tito_2YWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2YWJ-query.scw PDB file : Tito_Scwrl_2YWJ.pdb: