Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNGVLLLHKPVGMTSHDCVMKIRKLLKTKKVGHTGTLDPEVSGVLPICVGRATKIVEYLTEKSKTYDAEITLGFSTTTEDQTGETVETKPVNHDIDKADVEKVLNSLKGKQEQIPPMYSAVKVNGKKLYEYARAGIEVERPKRMITIEDIALTTEIKHHGETASFRFTVTCSKGTYVRTLAVMIGEKLGYPAHMSHLIRTASGDFSLDEC-FTFDELEAQA-------QSGTVEEHTVPIERALNHLPKWIISDTLAKKVENGALLETPEQFSEMT---SGDRIAVFT-ESGTCLAIYFPHPAKKGLLKPAKVLMQKSEQ 2AUS Chain:C ((45-305)) -QYGVINLDKPPGPTSHEVVAWIKRILNLEKAGHGGTLDPKVSGVLPVALERATRVVQALLPAGKEYVALMHLHG-------------------DVPEDKIRAVMKEFEGEIIQR-----------------------------TRKVYYIEILEIDG-----RDVLFRVGVEAGTYIRSLIHHIGLALGVGAHMAELRRTRSGPFKEDETLVTLHDLVDYYHFWKEDGIEEYIRKAIQPMEKAVEHLPKIWIKDSAVAAVAHGANLTVPGIVKLNAGIKKGDLVAIMTLKDE-LVALGKAMMS-----------------

General information: TITO was launched using: RESULT: Template: 2AUS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1222 -169725 -138.89 -716.14 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain C : 0.70 3D Compatibility (PKB) : -138.89 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_2AUS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2AUS-query.scw PDB file : Tito_Scwrl_2AUS.pdb: