Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFKFKKNFLVGLSAALMSISLFSATASAASTDYWQNWTDGGGIVNAVNGSGGNYSVNWSNTGNFVVGKGWTTGSPFRTINYNAGVWAPNGNGYLTLYGWTRSPLIEYYVVDSWGTYRPT--GTYKGTVKSDGGTYDIYTTTRYNAPSIDGDRTTFTQYWSVRQSKRPTGSNATITFSNHVNAWKSHGMNLGSNWAYQVMATEGYQSSGSSNVTVW 3AKT Chain:B ((14-189)) --------------------------------FYSYWNDGHGGVTYTNGPGGQFSVNWSNSGNFVGGKGWQPGTKNKVINF-SGSYNPNGNSYLSVYGWSRNPLIEYYIVENFGTYNPSTGATKLGEVTSDGSVYDIYRTQRVNQPSIIGT-ATFYQYWSVRRNHRSSGS---VNTANHFNAWAQQGLTLGT-MDYQIVAVEGYFSSGSASITV-

General information: TITO was launched using: RESULT: Template: 3AKT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1025 -10703 -10.44 -61.51 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -10.44 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_3AKT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3AKT-query.scw PDB file : Tito_Scwrl_3AKT.pdb: