Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MKIAVINGGTRSG-GNTDVLAEKAVQGFDAEH----IYLQKYPIQPIEDL------------RHAQGGFRPVQDDYDSIIERILQCHILIFATPI-YWFGMSGTLKLFIDRWSQTLRDPRFPDFKQQMSVKQAYVIAVGGDNPKIKGLP---LIQQFEHIFHFMGMSFKGYVLGEGNRPGDIL-RDHQALSAASRLLKRSDAI 1TIK Chain:A ((2-204)) SLSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLAANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIA-APMYNFNISTQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTFLGFIGITDVKFVFAEGIAYGP-EMAAKAQSDAKAAIDSIVSAE

General information: TITO was launched using: RESULT: Template: 1TIK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 830 -11235 -13.54 -62.77 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -13.54 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.426

(partial model without unconserved sides chains): PDB file : Tito_1TIK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TIK-query.scw PDB file : Tito_Scwrl_1TIK.pdb: