Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKLIEREKTVYYKEKPDPSSLGFGQYFTDYMFVMDYEEGIGWHHPRIAPYAPLTLDPSSSVFHYGQAVFEGLKAYRTDDGRVLLFRPDQNIKRLNRSCERMSMPPLDEELVLEALTQLVELEKDWVPK-EKGTSLYIRPFVIATEPSLGVKASRSYTFMIVLSPVGSYYGDDQLKPVRIYVEDEYVRAVNGGVGFAKTAGNYAASLQAQRKANELGYDQVLWLDAIEKKYVEEVGSMNIFFVI-NGEAVTPALSGSILSGVTRASAIELI-RSWGIPVREERISIDEVYAASARGELTEVFGTGTAAVVTPVGELNIHGKT--VIVGDGQIGDLSKKLYETITDIQLGKVKGPFNWTVEV 3UZO Chain:A ((25-358)) -----------------DWSTLGFSYIRTDLRYLAHWKDG-EWDAGTLTEDNQIHLAEGSTALHYGQQCFEGLKAYRCADGSINLFRPDQNAARMRMSCRRLLMPELSDEQFIDACLQVVRANEHFLPPYGTGGSLYLRPFVIGVGDNIGVRTAPEFIFSVFCVPVGPYF-KGGLTPTN-FITSDYDRAAPHGTGAAKVGGNYAASLLPGYEAKKRDFADVIYLDPATHTTIEEAGAANFFAITQDGQKFVTPQSPSILPSITKYSLLWLAEHRLGLEVEEGDIRIDEL------GKFSEAGACGTAAVITPIGGIQ-HGDDFHVFYSESEPGPVTRRLYDELVGIQYGDKEAPEGWIVKV

General information: TITO was launched using: RESULT: Template: 3UZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1887 -182603 -96.77 -555.02 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -96.77 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.479

(partial model without unconserved sides chains): PDB file : Tito_3UZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UZO-query.scw PDB file : Tito_Scwrl_3UZO.pdb: